DIN Standards Committee Food and Agricultural Products
DIN EN ISO 21528-2
Microbiology of the food chain - Horizontal method for the detection and enumeration of Enterobacteriaceae - Part 2: Colony-count technique (ISO 21528-2:2017); German version EN ISO 21528-2:2017
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren für den Nachweis und die Zählung von Enterobacteriaceae - Teil 2: Koloniezählverfahren (ISO 21528-2:2017); Deutsche Fassung EN ISO 21528-2:2017
Overview
This document specifies a method for the enumeration of Enterobacteriaceae. It is applicable to - products intended for human consumption and the feeding of animals, and - environmental samples in the area of primary production, food production and food handling. This technique is intended to be used when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample. The most probable number (MPN) technique, as included in ISO 21528-1, is generally used when the number sought is expected to be below 100 per millilitre or per gram of test sample. The following steps are carried out: 1. preparation of initial dilution and decimal dilutions; 2. isolation and selection of colonies for confirmation; 3. confirmation and 4. calculation of the number of Enterobacteriaceae. The responsible committee for this standard is NA 057-01-06 AA "Mikrobiologie der Lebensmittelkette" ("Microbiology of the food chain") at DIN Standards Committee Food and Agricultural Products (NAL).
Document: references other documents
Responsible national committee
NA 057-01-06 AA - Microbiology of the food chain
