DIN Standards Committee Food and Agricultural Products
DIN EN 16936
Animal feeding stuffs - Methods of sampling and analysis - Screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in compound feed by a microbiological plate test; German version EN 16936:2017
Futtermittel - Probenahme- und Untersuchungsverfahren - Screening auf die Antibiotika Tylosin, Virginiamycin, Spiramycin, Bacitracin-Zink und Avoparcin in Konzentrationen unterhalb von Zusatzstoffen in Mischfuttermitteln mittels mikrobiologischem Plattentest; Deutsche Fassung EN 16936:2017
Overview
DIN EN 16936 presents a method describing the screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in complete feeding stuffs and milk replacers by a microbiological 3-plate test. The limit of detection of the method is 1 mg/kg for avoparcin, tylosin, spiramycin and virginiamycin, and 5 mg/kg for zinc bacitracin. The presence of other (veterinary) antibiotics may interfere with the method. Furthermore, high concentrations of metals (Cu, Zn) may interfere. The method should be used as a qualitative screening method. Positive results can be analysed further by TLC; for confirmatory purposes LC-MS is required. The standard has been prepared by CEN/TC 327 "Animal feeding stuffs - Methods of sampling and analysis", the secretariat of which is held by NEN (Netherlands). The responsible German standardization committee is Working Committee NA 057-03-03 AA "Futtermittel" ("Animal feeding stuffs") at DIN Standards Committee Food and Agricultural Products (NAL).
Responsible national committee
NA 057-03-03 AA - Animal feeding stuffs
